TRIPHOSPYRIDINE NUCLEOTIDE-LINKED ALDEHYDE DEHYDROGENASE FROM YEAST
نویسندگان
چکیده
منابع مشابه
Diphosphopyridine Nucleotide - linked Aldehyde Dehydrogenase
In continuation of our investigation of liver diphosphopyridine nucleotide-linked aldehyde dehydrogenase (1)) we have undertaken a study of various classes of inhibitors of the enzyme. Interest in aldehyde oxidation has been increasing in recent years from several points of view. The classical problem of ethanol oxidation in viva still has many unresolved facets (Z), and the enzymes responsible...
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The dismutation of acetaldehyde to ethyl alcohol and acetic acid has been shown to occur in animal tissues (1, 2). The dependence of this reaction on diphosphopyridine nucleotide was demonstrated by von Euler and Brunius (3). Partial purification of the responsible enzyme system was achieved by Dixon and Lutwak-Mann (4). These authors clearly demonstrated that the mutase activity was independen...
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The potassium-activated, pyridine nucleotide-linked aldehyde dehydrogenase from yeast has been purified to the stage of homogeneity as judged by ultracentrifugation and gel electrophoresis. The enzyme has been crystallized, although this is not a recommended step in purification because loss of catalytic activity is thereby incurred. At least three separable, active fractions were obtained with...
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Coenzyme A-linked aldehyde dehydrogenase from Escherichia coIi strain B was purified 170-fold over cell-free extracts, and certain of its properties were investigated. The enzyme is essentially inactive in the absence of sulfhydryl compounds such as dithiothreitol and /3-mercaptoethanol. Addition of a thiol reagent after incubation of the enzyme with substrates results in very slow reactivation...
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The molecular weight of a DPN-linked isocitrate dehydrogenase preparation, exhibiting a single major band on polyacrylamide disc gel electrophoresis, has been examined by molecular sieve chromatography. Comparison of the elution volume (V,) of the enzyme with reference proteins of known molecular weight on columns of Sephadex G-ZOO and Sepharose 6B permitted calculation of a molecular weight in...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1953
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)66219-2